Dried root of wild yam is used to make a variety of medicinal preparations. Large amounts can cause vomiting.. Special Precautions & Warnings: Pregnancy and breast-feeding: There is not enough reliable information about the safety of taking wild yam if you are pregnant or breast feeding. (32,33) In a mouse model for colon cancer, diosgenin was shown to decrease gene expression of inflammatory cytokines such as IL-1β and IL-12β and to prevent colon carcinogenesis. Dioscorea japonica is a PERENNIAL CLIMBER growing to 3 m (9ft 10in). It increases the efficiency of Liver. This work was funded by JSPS KAKENHI Grant Number 24500948, ASHAHI GROUP FOUNDATION (Tokyo, Japan) and Ryobi Teien Memory Foundation (Okayama, Japan). 7). (1), (2), Research indicates that the disogenin found in wild yam extract may have a “depigmenting effect.” This means it could help with issues such as melasma, melanodermatitis and sun lentigo issues that eventually result in hyperpigmentation. Contents: About Nagaimo (Japanese Yam) Nutritional Value of Nagaimo Handling Nagaimo How to cook Nagaimo? already built in. The immunoreactive proteins were visualized using BM chemiluminescence Western blotting kit (Roche). Effects of Taiwanese yam (Dioscorea japonica Thunb var. It is in flower from September to October. It increases sexual drive in men and women. On the other hand, diosgenin did not have a significant effect on mPGES-1 expression. Dose-dependent effect of DJE on the expression of COX-2 and mPGES-1 in A549 cells. J Clin Biochem Nutr. Dioscorea japonica goes under several common names. DJE by 50% ethanol (0–100 µg/ml) was added to A549 cells. After the treatment with or without DJE by 50% ethanol (100 µg/ml) for 48 h, arachidonic acid (10 µM) was added to the culture medium of A549 cells. Wanasundera JPD, Ravindran G. Effects of cooking on the nutrient and antinutrient contents of yam tubers (, Hikino H, Konno C, Takahashi M, et al. Nutrition. 3.0 software (Fujifilm, Tokyo, Japan). Dioscorea japonica extract induced the translocation of nuclear factor-κB from the nucleus to the cytosol and inhibited the activity of the cyclooxygenase-2 promoter. Diosgenin (0.1–10 µM) was added to A549 cells. COX activity was analyzed as described previous report. Kawakami Y, Nakamura T, Hosokawa T, et al. Further, in human colon carcinoma model Caco-2 cells, treatment with DJE led to the suppression of COX-2 and mPGES-1 mRNAs to approximately 67% compared with the control (Fig. COX is the rate limiting enzyme responsible for the production of PGs and thromboxane from arachidonic acid. 4B). Each cell of the capsule usually contains 2 seeds (less often, only 1). The produced 9- and 13-hydroxyoctadecadienoic acids (HODEs) which cochromatographed on reversed-phase HPLC as a single peak were quantified by the comparison of the area of the peak with that of an internal standard, 15-hydroxyeicosatetraenoic acid (HETE). We also examined the possibility that D. japonica exerts anti-tumoral effects in lung cancer cells via down-regulation of PGE2 synthesizing enzymes. Addiction – What Are the Short- and Long-Term Effects of Drug Abuse? 2A). The plant is not self-fertile. After treatment with blocking reagent (Roche, Penzberg, Germany), the membrane was incubated with rabbit anti-COX-2 antibody (1:1000, Immuno-Biological Laboratories Co., Ltd., Gumma, Japan), rabbit anti-mPGES-1 antibody (1:1000, Cayman Chemical Co. Ann Arbor, MI) or rabbit anti β-actin anibody (1:1000, Cell Signaling Technology, Boston, MA). Male flowers are arranged in small clusters of 1-3 along the branches of each panicle. and Dioscorea nipponica Makino, in vitro. The values are represented as a relative value control cells and represent mean ± SD. Antiproliferative activity of guava leaf extract via inhibition of prostaglandin endoperoxide H synthase isoforms. In conclusion, based on the present results, we propose that D. japonica results in the suppression of COX-2 and mPGES-1 expression and ultimately inhibits the production of PGE2. Volume 64, Issue 17. (17) COX-2/Cluc plasmid with COX-2 promoter region and Cypridina luciferase (Cluc) as a reporter gene was transfected to A549 cells. More research is needed to determine whether wild yam would help people with high cholesterol. Ng SC, Chan FK. The cells were cultured in Dulbeco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 mg/ml streptomycin. It is famous for balancing female hormones and assists in the natural development of breast growth. It is valuable also in painful cholera morbus with cramps, neuralgic affections, spasmodic hiccough and spasmodic asthma. Thus, it may prove valuable in controlling inflammatory and metabolic processes as well as carcinogenesis. After treatment with DJE by 50% ethanol for 48 h, COX-2 and mPGES-1 protein levels were decreased to 1.37 and 0.82, respectively, compared with the control (2.67 and 5.58) in A549 cells (Fig. You may notice problems with 8 ounces chopped fresh wild yam root (or 4 ounces of dried). After converting total RNA to cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA), quantitative PCR was performed using Eva Green-Comparative Ct method (Bio-Rad). laeviuscula Alph.Wood, Dioscorea villosa var. D. japonica, a wild yam, is a relative of the Dioscoreaceae family native to Japan. R.Knuth, Dioscorea villosa subsp. Cluc reporter plasmid with COX-2 promoter was used for the promoter activity assay, as described in the Materials and Methods section (B). Dioscorea japonica feeding and Dioscorea japonica extract topical application suppressed the expression of cyclooxygenase-2, microsomal prostaglandin E synthase-1, interleukin-1β and interleukin-6 and inhibited tumor formation, hyperplasia and inflammatory cell infiltration. While there are over 600 species of wild yam, only 12 are edible. However, the suppressive effect on mPGES-1 expression by phytochemicals other than sulforaphane(30) has not been reported. It is in flower from September to October. Root, harvested in September, is used to make a homeopathic remedy. Laneuville O, Breuer DK, Xu N, et al. Known to cause headaches, menstrual irregularities & acne. It is usually consumed boiled, steamed, baked or fried. Arachidonic acid cascade and signal transduction. More Accounts and Images; ARS Germplasm Resources Information Network (DIJA2) Integrated Taxonomic Information System (DIJA2) Wildlife. Generating an ePub file may take a long time, please be patient. Yang DJ, Lu TJ, Hwang LS. 2A). Choi YH. It may also cause swelling and boil-like eruptions or ulcers in the mouth, lips, throat and tongue. Dioscorea japonica, known as East Asian mountain yam, yamaimo, or Japanese mountain yam, is a type of yam native to Japan (including Ryukyu and Bonin Islands), Korea, China, Taiwan, and Assam.. Dioscorea japonica is used for food.Jinenjo, also called the wild yam, is a related variety of Japanese yam that is used as an ingredient in soba noodles. Such inhibitors suppress tumor growth with decreasing the levels of the anti-apoptotic factor Bcl-2. (19,20) Similarly, the expression of inducible mPGES-1, preferentially coupled with COX-2, for PGE2 synthesis is enhanced in tumors. Datasheet. Traditional uses and benefits of Wild Yam, Wild Yam Recipe for Coughs, Spasms and Nausea. Watanabe K, Kurihara K, Suzuki T. Purification and characterization of membrane-bound prostaglandin E synthase from bovine heart. In some pathological conditions such as cancer and inflammation, NF-κB is translocated from the cytosol to the nucleus and plays a key role in regulating COX-2 transcriptional activity. Scale bar indicates 50 µm. Wild yam was used as a medicinal herb by the Mayans and the Aztecs, possibly as a pain treatment. Additionally the COX-2 promoter activity was measured using COX-2/Cluc plasmid with Cypridina luciferase reporter gene (Fig. Tanikawa N, Ohmiya Y, Ohkubo H, et al. Traditional medicine practitioners believe that wild yam has anti-inflammatory properties and use it to treat rheumatoid arthritis. Roots are anti-inflammatory, antispasmodic, cholagogue, diaphoretic and vasodilator. The quest for a food secure and safe world has led to continuous effort toward improvements of global food and health systems. It is hardy to zone (UK) 8. However, the other physiological effects of D. japonica have not been completely elucidated. Supernatants obtained from the centrifugation (10,000 × g) of lysates from A549 and Caco-2 cells were used as enzyme sources for measurement of COX activity. The aim of this study was to explore the novel functional effects of D. japonica on the regulation of the PGE2 synthesis pathway. 2A, 3B, 6A) or Dunnett’s (Fig. Several phytochemicals from other natural products, such as resveratrol,(27) humulon,(28) and chrysin,(29) have been shown to suppress the expression of COX-2. However, the effect of dioscoretine on blood sugar has only been researched in animals so far. Diosgenin inhibits Akt signaling and its downstream targets, NF-κB and Bcl-2, in human breast cancer cells. Root should not be stored for longer than 1 year, since it is likely to lose its medicinal virtues. It treats Intestinal disorders, Gallbladder Pain, Rheumatoid Arthritis and for increasing Energy. No potential conflicts of interest were disclosed. Bioassay-guided fractionation and chemical investigation of the extract of this yam resulted in the identification of two new withanolides, named dioscorolide A … . It is possible that wild yam might reduce this inflammation and have a positive effect on symptoms, but there is little evidence to support this. DJE suppressed the expression of COX-2 and mPGES-1 in a dose-dependent manner (Fig. After blocking with Block Ace (Dainippon Seiyaku, Osaka, Japan), the cells were incubated with anti-NF-κB p65 (C22B4) rabbit antibody (Cell Signaling Technology), and then were incubated with Cyanine (Cy) 3-labeled donkey anti-rabbit IgG (1:400, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). The powders were eluted with 20–80% ethanol (v/v, EtOH), and the extracts were immediately dried-up with nitrogen gas and were completely filling with the gas to protect oxidation. Indeed, selective COX-2 inhibitors, as well as other traditional non-steroidal anti-inflammatory drugs (NSAIDs), are used to provide symptomatic relief to patients with chronic diseases. hirticaulis (Bartlett) H.E.Ahles, Dioscorea villosa var. (1,2) Biosynthesis of PGE2 from arachidonic acid involves the enzymes cyclooxygenase (COX) and prostaglandin E synthase (PGES). HODEs metabolized from linoleic acid were stable and these are quantified by the typical reversed-phase HPLC analysis for low-molecular-weight lipids, although PGH2 synthesized from arachidonic acid is highly unstable and is immediately and non-enzymatically metabolized to several PGs. DJE by 50% ethanol (0–100 µg/ml) was added to A549 cells. 5B). Of all cancers, lung cancer has the highest mortality, and the 5-year survival rate is very low. Avoid use during pregnancy and breast feeding. The latter is inducible under pathological condition yam could be useful for cramps! 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