Nested PCR was used to test the blood or spleen of 89 TLCs for FcaGHV1 DNA; three (3.37%; 95% CI, 0.70–9.54) were positive. マイクロサテライト解析による結果です。, The Tsushima leopard cat exhibits extremely low genetic diversity compared with the Korean Amur leopard cat: Implications for conservation Percentages of variation explained by the first two axes were 53.27% and 7.36%, respectively. These data were obtained from row typing score of multiple genotyping experiments. Their estimated number in the wild is less than 100 (Izawa et al., 2009). Circle symbols: Korean population. Typos, corrections needed, missing information, abuse, etc. The Tsushima leopard cat (TLC; Prionailurus bengalensis euptilurus) is a critically endangered species that lives only on Tsushima Island, Nagasaki, Japan. Footprints Round shaped footprints with four finger pads, and with no claw marks. Genetic diversity indices of two regional populations of the Amur leopard cat with 12 microsatellite markers. The Tsushima leopard cat population has a lower genetic diversity in nuclear DNA, as evidenced by molecular genetic analysis, than does the regional Korean continental population, which belongs to the same subspecies: the Amur leopard cat. Other than the Tsushima leopard cat, there are several kinds of mammals such as Tsushima deer, Tsushima martens, and Korean weasels. The Tsushima leopard cat is a regional population of the Amur leopard cat (P. bengalensis euptilurus)—a subspecies of the leopard cat (P. bengalensis). Therefore, if you see a cat in the wild with white spots of the back of its ears, you can be pretty certain that you have seen a Tsushima leopard cat! 1. Because the present study involved the comparison between the two regional populations that belong to the same subspecies, the bias is considered to be smaller than the bias observed in comparison between different species. Clustering of the two populations observed in the STRUCTURE (Fig. The captive population of Tsushima leopard cat has 21 founders that introduced to captive population from wild in 1996–2015. The Tsushima population showed lower molecular genetic diversity, as represented by number of alleles, heterozygosity, and allelic richness, than did the Korean regional population (Table 2). Biological research has been conducted; for example, radio-transmitters are used to track and understand their behavior and ecology. Females are slightly smaller than males. In 1995, the Japanese Ministry of the Environment established the Protection and Breeding Program for the Tsushima Leopard Cat. If you continue to use this site we will assume that you are happy with it. and will receive updates in the daily or weekly email digests if turned on. Their estimated number in the wild is less than 100 Moreover, we evaluated genetic differentiation between the two populations using structure analysis and principal coordinate analysis (PCoA). In recent years, genome-wide analyses have been reported to clarify the extent of gene flow and divergence among populations (Prado-Martinez et al., 2013).

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